FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2576_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2576_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452193
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19010.42039571598852704No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC10500.23220173686899181No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG7650.1691755511474083No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCTA200.00695875552.4819910
TGTTAGG400.002421957434.987992
CACGCAC450.00429895831.12110357
CCCAGAC450.004312956231.1004372
CCCGTGG450.00431529331.0969966
GTATAGG604.9766543E-429.1695751
ACCTGGG500.007221793327.9872936
CCAGCGT500.007225695527.9841984
CGTATCA2550.026.1012670
CGCCGTA2550.026.09836867
GCCGTAT2650.025.1107468
GCTTAGA800.002663123421.877181
TTAGGTA800.002673412221.8626584
GTATATA1957.748895E-1021.5406071
CTATCCA1003.943538E-420.988154
TCGCCGT3400.020.60397566
GGGTATA850.003785068620.5902861
GTCCAGG850.003785068620.5902861
CGGCGAT1107.468069E-419.09069815
TCACTAC1107.495927E-419.0801373
Produced by FastQC (version 0.11.2)