FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2572_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2572_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283320
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC8700.30707327403642526No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG6840.24142312579415504No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5890.20789213610052237No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTA350.001256378339.985685
AGCGCGC400.002420615434.98747310
GTCTAAC552.9786155E-431.8348831
TATGCTA450.00431058331.0999725
TACCCTC500.00721394327.9899755
GTTCGCG500.00721394327.98997511
GTATTAT1151.4843808E-624.3606051
CATAAGC750.001827812223.33733745
CGCCGTA1154.088845E-521.31552967
TTCTAAG1154.10559E-521.3042412
CTCCGTG850.003791679920.5808647
CGTATCA1257.768409E-519.61028770
GCCGTAT1257.768409E-519.61028768
TGTATAC900.005272779619.4409123
TACAGAT900.005278164519.4374837
ATGTAGT950.0071757618.4307231
GCTAAAT950.0071757618.4307231
TCGTCAC950.00719773418.42096114
TTCTTGG1150.001001928118.2704541
TTATACT1351.4115554E-418.1416514
Produced by FastQC (version 0.11.2)