FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2567_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2567_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225060
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8310.36923487070114636No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC3560.1581800408779881No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG2710.12041233448858081No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTAT450.00429413431.12151
GGCGTGG450.00430350231.10764910
GGGCGTG450.00431288631.0938119
CTTGCTA450.004317583531.08689730
TCTGGAC450.004317583531.0868973
CGTATCA1401.2732926E-1130.03007370
GCCGTAT1401.2732926E-1130.01669968
GTACATG703.470564E-530.0100171
CGCCGTA1502.910383E-1128.01558767
CTGTCCA500.00721780227.9844289
CTGATCG500.00721780227.9844289
TCGCCGT1606.366463E-1126.26461266
GTCGCCG1659.458745E-1125.46871665
ATATAAG700.001221104625.0083471
TGGTCGC1701.364242E-1024.71963563
CCGTATC1751.9645086E-1024.0187169
GGTCGCC1751.9826984E-1024.0133664
GTGGTCG1957.6397555E-1021.55045162
GCTTAAG1003.9053513E-421.0116962
TCTGTCC850.003798523520.572218
Produced by FastQC (version 0.11.2)