FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2562_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2562_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820361
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26110.3182745157314889No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA3900.029.62915867
GCCGTAT4200.027.51278768
TACAGGG700.00123079324.984162
CGTATCA5000.023.11215470
TCGCCGT5050.022.88192466
GGTCGCC5200.022.22322564
GTCGCCG5600.021.25988265
GCGAGGC1003.9264455E-421.00463316
TGGTCGC5750.020.09875163
GATGTAG1408.278686E-619.9909881
TAGTACC1408.304918E-619.9836714
CGGTGGT5850.019.75518460
GTGGTCG6050.019.68097162
TCTATAC2053.2641765E-818.7674453
GGTGGTC6550.018.17860861
CATAGGC1551.9807489E-518.067439
CCGTATC6450.017.91530469
CTCGGTG6550.017.64394458
CCGCAAC1000.00971665317.49104729
GTCCTAG3650.017.2524951
Produced by FastQC (version 0.11.2)