FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2559_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2559_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333240
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16740.5023406553835074No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGT200.006965783452.4655464
CTATAGG352.0886477E-549.9821971
CTAAGAT350.001259470639.967753
GTCAAGC400.00241585335.00330717
GTTCACG501.7099493E-434.9927911
CCTAGAC400.00242655734.9717833
ATAGTGG501.7159987E-434.971783
TATAGGA552.997814E-431.8020742
CGGCCAA450.0043084531.10470411
CGTATCA1950.028.73361470
CGCCGTA2000.028.0110667
GCCGTAT2000.028.0110668
CCCTTGG500.007215666627.9900281
CGGTCGC500.007215666627.9900289
ATGGGTG500.00722625527.9816235
TAGGCTC500.00722625527.9816235
CAATAAG1154.899448E-827.381551
CAATGCG657.9639535E-426.913499
TCTAGTA700.001228345724.9873432
TCGCCGT2400.023.3425566
Produced by FastQC (version 0.11.2)