FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2556_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2556_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321419
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6210.1932057532379853No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC5800.1804498178390201No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG4510.14031528938861737No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA305.870916E-446.67009453
GTACTGG604.965189E-429.1778951
GTGTTAG500.007189230528.010781
AGTCTGC500.00722209827.9846256
TGGACAG700.001228558624.9862755
ATACTAA851.3110331E-424.6923166
GTATAAC750.00182607423.3423181
GTCTTAT750.00182607423.3423181
GCTTTAT750.00182607423.3423181
GTATTAC901.9196488E-423.3423161
TCTTAGC750.001834365723.3241528
GGCATAA850.003783295920.58974855
CTGTTAA850.00379697220.57693134
TAATCCG850.00379697220.5769315
TATTACA1408.215666E-620.0014692
CGTTATG900.005257036519.4519311
CGTGTCA900.00528551219.43376734
CTAATAC1107.480878E-419.0833973
TTTATAC1702.118075E-618.522123
GTATATA1551.9708314E-518.071471
Produced by FastQC (version 0.11.2)