FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2555_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2555_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308715
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12850.416241517257017No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC5680.1839884683283935No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG3950.12794972709456942No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGCGG450.004322882331.0827431
GTATTAC657.958331E-426.915971
GTATAGA700.001226406524.9933991
TATACCG700.001231051924.9772015
TAGACCA750.00183974623.3120565
ATATTAG1055.4638286E-419.994721
TATTACA1601.1891862E-619.6727332
GTGCACA900.005291193319.42986111
TATACCC900.00529615419.4267125
GTATATA1301.0565879E-418.8411791
TAAAGGT1301.0618587E-418.8289664
TTATACT1301.0618587E-418.8289664
TGTTATA950.00723017618.4072362
AACCGTC950.0072369418.4042537
CGTATCA1752.7596652E-618.01569470
GTATAAT1602.6033062E-517.4953781
CAATACT1200.001346263917.4840414
AGCAGTG1000.00972574217.484047
ATCCAGC1250.001769419816.784686
CGCCGTA1906.0329585E-616.59609667
Produced by FastQC (version 0.11.2)