FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2554_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2554_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332147
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15430.46455334535612247No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC9180.27638364940824395No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG6630.19961041346150948No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCCC200.00695711452.48202534
TTACACG305.8876176E-446.6436584
GTCCTAA459.206301E-538.8755721
ATAGGAC400.002422815434.9827423
GTATAGG552.994909E-431.8072871
TACAGGT700.001228241724.9876734
CTTACCC700.001228241724.9876733
TATACTT1001.4002055E-524.4879195
TTACCCA750.001835554823.3218294
GGCATAA1102.9196923E-522.26845749
GTATACA952.7820683E-422.0976941
TAGGCTG800.002670823421.8642145
CGCCGTA1807.188646E-921.39769267
CTGTCCC850.00379268120.5811869
CGTATCA2051.4388206E-920.49314570
GTTCTAG1055.4679107E-419.9931531
TAATACT1055.47347E-419.9901394
AGAACCG1055.47347E-419.9901395
ATATACT1257.834597E-519.5903364
TATAGTA900.00527035519.4436472
Produced by FastQC (version 0.11.2)