FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2547_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2547_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370604
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14620.39449115497943893No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC5670.15299349170543222No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG4550.12277255507226043No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCGT350.001258195139.977168
GTGCGAC500.007219507427.98779711
TATGGAC500.007229031527.9802383
CTTAGAG500.007229031527.9802383
GTCCTCA657.9756574E-426.9077076
TAGGGGA657.9819513E-426.9040764
CGTATCA1900.025.80261870
GTATAGA959.4170555E-625.774751
GTATTAA851.3117556E-424.6917781
GGAGACC750.001831023723.33261314
ATGCCAG750.001833904123.32631128
GTATTAT1352.3185203E-723.3200111
CGCCGTA2207.2759576E-1222.2870967
ATAGGCA952.7745176E-422.10756546
CCTATAC800.002674709321.859563
GCCGTAT2259.094947E-1221.79182468
TAGGCAT2108.367351E-1121.66892447
GTACTTA1003.9418577E-420.988011
GTATAAC850.003798530420.5764831
AGGCATA2252.0918378E-1020.2243348
Produced by FastQC (version 0.11.2)