FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2544_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2544_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304316
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9110.2993598759184532No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC5290.1738324636233389No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG3810.12519880650376583No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG150.002218374770.03934564
GCGATTA200.006945269652.5035852
GTACCAT450.00431944231.0876546
TAGGTCA500.007205546427.99730138
ATAGGTC700.001225166724.99759137
GTGTATA1202.1558499E-623.3387641
TGTAGAG1052.046143E-523.315742
AAGACAG901.9385145E-423.3080755
CCCTAGT2350.022.3235822
CGCCGTA1102.9017092E-522.28524867
CCCCAAG850.00377934420.5930271
ACCTGCT850.003801023420.5727127
CCGCATT1205.6897738E-520.42141715
GACAGTC1257.847584E-519.5852227
GCCGTAT1301.04984676E-418.85674768
TCCGCAT1301.05250314E-418.8505414
TTAATAC1301.0618463E-418.8288483
AGGTTGG950.007230106718.4071647
TATGGCT950.00724383818.4011124
CATAAGA1953.936857E-717.94403651
Produced by FastQC (version 0.11.2)