FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2542_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2542_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283038
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8820.3116189345600237No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC6500.22965114224945063No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG4420.15616277672962642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGTG556.718614E-638.1508565
GCCACGA400.002419685234.9901836
TAAAGGA501.7153898E-434.971624
CATTAAG450.004294024331.12440960
AGCGATC450.004316431.0913818
TACCCGA500.00721118527.99214436
ATTAGGG500.007217417527.9871923
TATTAGG700.001226270624.9929872
GAGCAGC901.9344775E-423.3144116
TTATAGT952.789492E-422.0873374
GATTAAG800.002652889621.88822668
CTCTTAA1003.9309537E-420.9941081
CCTTGTT850.003789954120.5824582
TTTAGAC900.005281169419.435553
TAGTAGT900.00529198419.4286774
AACCTAA950.007179754318.42892666
GTATAGA950.007209149718.4158841
GTCTGCT950.007216513618.4126269
ACTAGAC950.007216513618.4126263
GCATAAG1000.00964905117.5074856
Produced by FastQC (version 0.11.2)