FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2525_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2525_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342110
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10760.31451872204846393No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC7690.22478150302534272No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG4970.14527491157814737No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGT450.00431334531.0977234
TATAGTG901.7592174E-731.0977235
TAAGGCG450.00431334531.0977235
CTACACT500.00721854727.9879534
ATAGGGC700.001227869724.9892433
CGGGTTC850.003794849920.5793786
GTAGACA1055.4716284E-419.9913944
TAACGAG900.00528256319.4360775
TTATGCT1107.484079E-419.0826934
CTGTCCA950.007206232318.4185129
TTAACCT950.007218405618.4131264
TATAATA1602.5985548E-517.5001472
TCGTACA2207.2275725E-817.50014521
CATAGGC1803.648036E-617.49758739
GTATATG1200.00133969517.4975871
GGGTTCT1000.00970087317.4924727
TTAAACC1000.00970087317.4924723
CATAGAG1200.001342324717.492473
CGTATCA1854.703421E-617.03713870
ATGTAAT1653.3884426E-516.9673581
Produced by FastQC (version 0.11.2)