FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2523_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2523_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322096
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11720.3638666732899508No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC9650.2996001192191148No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG6770.21018578312056035No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGT401.0668373E-861.2282454
CGCGAGT200.006940187452.51399622
TATACCC404.597025E-543.7344634
AGCACTA350.001257575939.9795765
TGCAGTG400.00241922334.99300812
AGTAACG450.0043048331.10972826
CTAAGCG450.004311384631.1000613
CTCTAAG703.4841716E-529.9986651
CTTAATA604.9867725E-429.1563072
GGGTGTT604.9867725E-429.1563077
TCTAAGC604.9867725E-429.1563072
CTGTTAG500.007204334727.9987581
GCATAAG1251.09388566E-725.20279956
CTACTCC700.001227174724.9911176
ATACCCA750.001835622223.321425
GTATAGA800.002663699721.874031
CAGTCCA800.002666105321.870639
ATAATAC1506.377704E-720.992543
ATAGTGA1506.377704E-720.992546
TAATACT850.003792720120.5809234
Produced by FastQC (version 0.11.2)