FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2521_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2521_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349872
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10630.303825399003064No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC6690.19121278639045136No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG4840.1383363058489962No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCA200.00695020352.4958239
GTATAGA808.658448E-526.2479111
TTAAGGT700.001226439524.9944364
AAAGCCT1502.4680048E-823.33147640
CAAAGCC1953.092282E-1123.33147439
CATATAC800.002664706421.8732591
TCTATAC1604.897811E-821.8701323
TAATGGT1304.3390082E-621.5336694
GTATGTG850.003787319420.5865969
ACTGAGC850.003790457520.5836528
TATGGTG1205.7129186E-520.4121255
CGCCGTA2101.9426807E-920.00698767
TGTATAC1257.819881E-519.5956383
GATACAC900.005276460719.4401175
CTATACA900.005276460719.4401174
GCCGTAT2254.5765773E-918.67318768
ACAGTGC950.00721008318.4169528
CGTATCA2306.009941E-918.26724870
ACAGTGT1150.001007615718.2568058
TAGTGAT1150.001007615718.2568057
Produced by FastQC (version 0.11.2)