FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2516_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2516_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141351
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5490.38839484687055625No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC3270.23133900715240785No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG2280.16130059214296327No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTAG200.00694906352.4816671
TTACACT350.001258560639.9577264
ACGGGAA400.002420615234.9753935
CAAGTAC400.002424825434.963013
TATCCTT450.004318088331.0782325
GGTCGCC703.4525256E-530.02142764
CGCCGTA703.4525256E-530.02142767
TGGTCGC703.4525256E-530.02142763
CGTATCA755.5269244E-528.0270
GTGGTCT500.007201561727.99022512
AAGTACT500.00722651927.9704064
AGGCGTG500.00722651927.9704067
TATAGTG500.00722651927.9704065
TGTGATG657.940792E-426.91367512
GTGGTCG808.576098E-526.26875162
GCCGTAT808.576098E-526.26875168
TTGTATA700.00122371924.991272
TACACTT700.001228785824.9735775
TCGCCGT901.9079674E-423.3566
GTCGCCG901.9079674E-423.3565
Produced by FastQC (version 0.11.2)