FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2514_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2514_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235919
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8400.35605440850461384No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC2770.1174131799473548No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCC200.006961049552.4697849
ACCGTGC200.006961049552.4697848
TCCTAAG459.199341E-538.8747522
TAATACT652.1052507E-532.2890974
TCTAAAG703.487854E-529.9890942
CCTAAGG604.989215E-429.149883
TATTATA1253.5433914E-927.989822
ATACTAT500.00721919427.9838836
GTTGTAC500.00721919427.9838833
GCACTGC500.00721919427.9838836
GTAGAAC657.9672347E-426.9075813
GTAGTAG700.001223229925.0015161
ATTTAGG851.304173E-424.707381
GTATATA851.304173E-424.707381
GTATTAT901.921759E-423.334751
TAACACT901.9300682E-423.3199044
GTGAGTA750.00183486823.3199049
AACACTG1003.9361254E-420.9879135
TAATCTA850.003794600720.5763874
TTAACAC900.005282230719.4332543
Produced by FastQC (version 0.11.2)