FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2512_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2512_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162395
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4730.29126512515779424No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCCGT200.006945438752.49213827
TACGGCT200.00695391552.4759564
TACTCAT350.001253505439.9940077
CACGTAA400.002407802535.0163541
GCCCACT400.002411451235.00555413
TACACCG400.002418761434.983975
CGCCCAC500.007186829628.00444212
TAAGTAT500.007208511227.9871774
ATGAGCC657.9366704E-426.9190449
GTTAGAA700.001220884625.00396325
ATTACAT750.001831156123.3226493
TATACTC952.7757522E-422.095145
CGTATCA800.00265492521.87847170
CGCCGTA800.00265492521.87847167
GCCGTAT800.00265492521.87847168
GTCGCCG850.003773471620.59150365
CTTCTAC900.005243533819.453531
TGGTCGC900.005252900519.44752963
TATTACA900.00526228119.4415322
GTTTTAC900.00526228119.4415326
Produced by FastQC (version 0.11.2)