FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2511_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2511_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259763
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7750.2983488795555949No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC5430.2090366988370168No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG3650.14051269811328018No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGCC150.00222840369.956838
TAACCTA400.002427901634.964945
AGGGGGC450.00430318231.10991324
CTTAATG700.001232553224.9701463
CGTTCAG750.001835801423.31894510
TATGGGT750.001841986923.3054714
CGCCGTA1703.6852725E-922.66804167
ATACAAC800.00268016821.8488793
GGTCGCC1807.1286195E-921.40870564
TTATACT1154.1417097E-521.278914
GAGTTAA850.0038007620.5715751
GCCGTAT1951.794615E-819.7580768
GTGTTGC900.005290782519.4287091
CTTCTAG900.005290782519.4287091
GTCGCCG2002.3948814E-819.26783465
ATTATAC1107.5159874E-419.0681113
GACTATT950.007229625718.4061457
GAAGGGT950.007229625718.4061456
GTTAATC950.00724571918.3990553
CGTATCA2104.1896783E-818.35031970
Produced by FastQC (version 0.11.2)