FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2501_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2501_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183503
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5230.28500896443109924No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC2010.10953499397830008No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGCA200.00693066952.5231422
TGCCGTT200.006953151452.4801611
GGACTAC200.006968169552.451546
AGGTAGG305.893179E-446.6235926
GCATTGT450.004301322631.10783638
TGTTCGG450.00430707131.09935210
TATGTTA450.004318587531.0823965
GTTACTC450.004324354731.0739213
GCCGTAT703.451673E-530.02961768
TGTATAC500.007227333327.9741552
TATCCAG500.007227333327.9741555
CCAGACG500.007236961327.966533
TAAGGAG500.007236961327.966533
ACGCAAT657.92592E-426.9275915
TACGCAA657.938573E-426.92024214
GTGTTCG657.9639297E-426.905569
CTAGACT657.976633E-426.8982264
CGCCGTA808.5739186E-526.27591567
ACATACG700.001223373324.9973738
GTATAAA700.001225321724.9905511
Produced by FastQC (version 0.11.2)