FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2499_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2499_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550070
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26850.4881196938571455No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC12970.23578817241442No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG9870.17943170869162106No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTGG605.0064886E-429.1411936
GTGTATA1554.3655746E-1127.085431
CGCCGTA3400.025.75734167
CGTATCA3450.025.38635870
AGAACCG1151.5104579E-624.3199235
GCCGTAT3700.023.66675268
GCACCGC750.001841154223.3129546
CTATACT1202.188941E-623.3044744
CCTATAC901.9457172E-423.3002363
GGTCGCC4150.021.102464
CAGTGCA1003.9508508E-420.9835689
CTAACTC1356.0895964E-620.7113233
GTTATAC1356.0895964E-620.7113233
GTCGCCG4400.020.69953565
GCTCTAG850.003797483620.5796171
TATACTC1751.27784E-719.9770815
TCGCCGT4800.019.7025766
GGCCATT1257.831104E-519.5953611
TAGACCC900.00531129519.4203974
TAGAGTC1107.528176E-419.069035
Produced by FastQC (version 0.11.2)