FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2498_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2498_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495345
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16720.3375425208692931No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC7110.1435363231687006No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG5150.10396794153569734No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAGC450.004301457731.11798715
CTTACCG657.9708244E-426.912710
GTATAAG1305.4405973E-926.9018251
TACTGTG1202.1777905E-623.3172727
GTTTAGC1003.9502705E-420.9834231
GTACAGA850.003805127520.5719831
ACAGACC1055.482186E-419.9882538
CTTGGAC1055.493396E-419.9821953
CCTACAC900.00529992319.4271343
CTGTGCC1107.488287E-419.0835519
GGTTCCG1107.51378E-419.0739145
GTCCATA1301.06250736E-418.8312781
CGCCGTA1501.4869262E-518.67645868
TCTATAC1501.5051724E-518.650053
GTACCGT950.00723784318.4065136
TACAGTG1551.9926643E-518.052087
GGATAGG1000.00972692517.4861871
GTATATA1653.4124245E-516.9563031
ACAGTGT1704.39985E-516.4609138
GTGATAC1300.002284944316.15741525
Produced by FastQC (version 0.11.2)