FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2497_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2497_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476001
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24050.5052510393885727No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC12660.26596582780288275No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG9860.20714242196970176No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACT501.7181651E-434.9680944
ACTGGCC808.689174E-526.2371038
CGTATCA2850.023.3488870
CGCCGTA2550.023.34642467
TTAGGAC750.001840075223.3145123
TATACCG750.001841201223.3120615
GCCGTAT2800.021.26192368
CTATACC1205.7488825E-520.3980544
GTCGCCG3000.019.8444665
TCGCCGT3050.019.51914266
AATGCGC900.005287404619.4348930
AGGCATA2353.783498E-1019.35829748
GGCATAA1451.1176218E-519.30491849
TATACTT1852.2868335E-718.901675
GTTATAT1301.0556974E-418.8468061
ATAGGCA1501.48895815E-518.67320646
TATAAGA1501.4984416E-518.6594582
GGCATAG1702.1073847E-618.53590443
ACGCCTC950.007189984418.42750721
GTAGTAA950.007203095618.421691
Produced by FastQC (version 0.11.2)