FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2496_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2496_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482528
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC18460.38256847271039196No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG14940.30961933815239734No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14070.29158929637243847No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGG500.007226479227.9839538
TATACCG500.00723013727.9810545
AGTGCCG657.9792767E-426.9076488
CTTATAG700.001230368424.9830823
ACCGTTC901.9300406E-423.32721736
TATACTT2004.3655746E-1122.7346065
ATGCGAT800.002668983821.86926511
GAGCGTC800.002675417821.8601977
TCCCTTG1003.9415417E-420.99014329
AGTGTAC850.003800221520.5764378
CTTGAGG850.003802504620.5743033
CAGTACT850.003802504620.5743034
TATATCG1408.283276E-619.9864655
TATAGTG1055.48521E-419.9864655
ATCTAGT900.00526154219.4514481
CCCACAG900.00528684519.4353169
CACCGTG900.00529318619.4312887
TAATACA2254.647518E-918.6540344
CGCCGTA1150.001003730318.26934268
TAACAGT1351.4176218E-418.1358664
Produced by FastQC (version 0.11.2)