FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2495_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2495_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405783
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC20900.5150536123987451No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG15500.38197755943447603No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9480.23362240409282795No Hit
CTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTT7280.17940623436664424No Hit
GTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAA5970.14712296966605304No Hit
GGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAA5960.14687653253093402No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGTAG400.002425798234.9758345
GACCCGG450.004298965431.12032955
TTAGCGA450.004314569831.09729811
GTTAGCG450.004314569831.09729810
TATTACG450.004314569831.0972982
TATAGGA802.471952E-630.6114032
AGTCCCC500.007220588627.98756810
TGTGCCC500.007220588627.9875689
TCGGCGC500.007220588627.98756830
TACGGTT500.00722493827.9841186
CTTACGG500.007233642527.9772174
CTATTCG1207.390372E-826.2480551
GTACTAA1052.0375734E-523.3316061
GGCATAA2108.367351E-1121.67308443
GGCTTAA1154.1016727E-521.31065855
GACTAAG850.003769298520.60450661
CCGGTCA850.003790927120.58417115
CACATTA850.003801779620.57401831
TTACACC850.003804496720.5714824
GATTAAG1558.675197E-720.3336268