FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2491_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2491_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311138
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14720.4731019676156561No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC5970.19187627355064313No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG4150.13338132918512044No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGG200.006938382552.51702563
CTACCCG305.8820035E-446.651764
TATAGGG450.00430362231.1111812
CTCCTAC703.4788543E-530.0048981
AGTAATA604.975956E-429.1667352
GCCACAG500.007196659728.0045721
CCTTCGC500.007219315527.986558
CGCCGTA1951.8189894E-1225.14445167
CTGCACA700.001226824624.9920124
TCCTACA851.3059973E-424.705942
GTATTAA1052.0318546E-523.3371431
GCCGTAT2155.456968E-1222.80543368
GGTCGCC2259.094947E-1221.78484264
CGTATCA2301.2732926E-1121.32155670
CTGAGCA1154.122329E-521.2941159
GTGGTCG2501.8189894E-1221.0068162
TGGTCGC2351.6370905E-1120.85782663
GCATGGA850.003781052820.5915971
GTAATAC850.00378811320.584973
TAATACT1205.7145502E-520.4101454
Produced by FastQC (version 0.11.2)