FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2487_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2487_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146022
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC5670.3882976537781978No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3750.25681061757817314No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG3280.22462368684170878No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG150.002224714369.970529
AAGGCGC200.006960942452.4598850
TTAACAC200.0069798552.4239233
GTATAAA450.004326857631.0660291
CCCACAA700.001221978324.9980413
AATAATG800.002673235521.8507882
TGAACAT850.003791903420.572510
GTTTTAG850.003807006420.55841
CTTTGCT900.005278511419.4295839
TGAAGTG1107.5073034E-419.0632445
TCTTATT950.007227229418.4006632
CATTAAA950.007241545218.3943581
TTAGGTG2305.9062586E-918.27197870
TTAAACA1150.001012093718.2344074
CTAGTGA3700.017.9469264
GAATAAT1401.8722782E-417.474641
AGTGACC3850.017.253616
TAGTGAC3700.017.0023525
CCTAGTG3156.184564E-1116.6425133
CCCTAGT3207.8216544E-1116.3880922
Produced by FastQC (version 0.11.2)