FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2484_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2484_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369145
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC9540.25843503230437903No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8640.23405436887943762No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG6810.18448035324872342No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA450.00430172131.11551360
TACGGGT450.004316024531.0944184
CTATACC703.4972727E-529.9839044
CTGTGCC500.007213459427.9925679
TCTATAC755.5880264E-527.9925673
TAATGTG755.5983663E-527.9849765
GTACTGG700.001222995525.0069311
CGCCGTA1202.1570904E-623.33980267
GATGTGC750.001833510223.3271396
GTATTAA952.7712405E-422.1113931
TCTACTA1154.113871E-521.3015822
TGCGGAG1003.9264868E-421.00012212
CACAACG1003.9336877E-420.9944273
AGTCTAC850.00379178120.5827718
GTTCTAG1055.4467015E-420.0055451
CGTATCA1408.206611E-620.00554570
GGCTAGA900.005261759319.4498351
CATAACG1301.0540149E-418.8488224
GGTCGCC1501.49015905E-518.66930864
GGCATAA1501.49015905E-518.66930843
Produced by FastQC (version 0.11.2)