FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2480_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2480_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185352
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5440.29349561914627303No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC4050.21850317234235397No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG2680.14458975355000214No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGAC350.001255707839.9827087
TAATACC400.002422544934.9754184
ATGATGC500.00721019427.9878968
CGCCGTA750.001823227723.34216767
ATAATAC952.7874962E-422.083773
TACTATT800.002665463421.8655437
CCAGAAC800.00267384121.8537353
GTCTAGA1154.1006602E-521.3008921
CGGTGGT850.003764740220.601660
GCCGTAT850.00377064720.5960368
CTAGACT1205.7153877E-520.4023284
TTATACT1205.7153877E-520.4023284
ATTATAC1205.727292E-520.396823
TCTAGAC1408.259169E-619.9805563
GTCGCCG900.00524896719.45180765
TATACAT900.005281895419.4307885
CTCAACC950.007183835418.42304420
ACTCGTT950.007183835418.42304419
GCCAAAG1150.001001121618.26778443
GGCATAG1150.001006591618.25297531
Produced by FastQC (version 0.11.2)