FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2478_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2478_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110060
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4450.4043249136834454No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC2300.20897692167908413No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG1320.11993458113756132No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCAG150.00222257769.975916
TCGCTAC150.00222257769.9759114
TACATCG200.00694486752.481935
CTGCTCG404.57692E-543.7349439
TAAGAAC350.001252944539.9862333
AACAGAC450.00429894231.1004057
TCCGCCC500.0071945827.99036450
CTACTCT500.0071945827.9903644
GAACAGA700.001221964624.9913966
GGTGGTC800.002643288621.8873761
GCGTATG900.005230035619.45543919
AGAACAG900.005257637719.4377525
CGTATGT1159.96354E-418.27119420
TGTACGA1000.00963027117.50193237
TTGCGTA1000.00965553817.49397717
ATGTACC1250.001742341916.80949823
TACCCCT1250.001752989516.79421826
CTTACGT2301.1710654E-716.74859670
TAGGGGT2108.44082E-716.660934
ACTAGGG2151.0793337E-616.2734682
Produced by FastQC (version 0.11.2)