FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2477_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2477_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188390
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5580.29619406550241517No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC3810.2022400339720792No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG2930.15552842507564096No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGCT150.002223344569.9893712
TAGCAGT200.006961838352.4641464
GTAATAC305.8943185E-446.622413
TAGCTAG350.001254030639.99392326
CGTGGCT450.004302592531.1063868
ACGATGT450.004302592531.1063868
GTATATA500.007200627627.995751
TTATGTA500.007219338327.9808754
AATGCGG657.9415296E-426.91898710
GTCTTGA700.001223828124.9962021
TCAAAGG750.001837484423.3112073
TAATCAT800.002661290621.8716827
TGCGGAG850.003782493320.5851112
ATGCGGA850.003782493320.5851111
TGTACAG850.003794161620.5741735
TTTGTAC850.003800006820.5687123
ACAATGC1257.791145E-519.5970258
TCTAGGA900.00528163119.4311662
GCGGAGA950.007195068518.41825513
AACAGTC950.007206112618.413367
Produced by FastQC (version 0.11.2)