FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2476_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2476_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260632
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC12480.4788360600386752No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG9010.34569814911446023No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5680.2179317965560637No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCGT200.00693901452.51340514
TACGCAT200.006944290452.50331547
TGGCAGC350.001252405840.01021612
AGCCATA400.002415258735.00221320
GTTCTGC501.7120152E-434.982041
GGCATAA1551.8189894E-1229.35669143
GCTGAAG604.966397E-429.17411468
CCGATCG500.007191311628.0071513
GGGCGGT500.007191311628.0071516
GTATTTA657.9665665E-426.9092641
CTCCGCA1056.7278415E-726.67347513
ACAGGTC700.00122355525.0015846
TTAAGCA750.001828568323.33480652
GTGTCAT750.001828568323.33480617
CCTAAGA901.924982E-423.33032250
CAACGTT901.9274908E-423.325845
CATAGGC1652.6484486E-923.3258433
GCCAAAG2155.456968E-1222.79213543
GGCATAG2004.3655746E-1122.74269531
AGCCAAA3450.022.3202542
Produced by FastQC (version 0.11.2)