FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n02_qg2475_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n02_qg2475_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169126
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC5410.31987985289074417No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG4490.2654825396449984No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4080.24124025874200297No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGC200.00694995952.484472
GTCCATG350.001256383339.9763371
CGCCGTA459.136668E-538.91192267
TGGTCGC501.7013618E-435.0103663
TGGACAG400.00241712434.9896475
GCCGTAT552.9775567E-431.82760268
CGTGCGA552.9827346E-431.81818422
GCGTGCG552.9827346E-431.81818421
TCCGATA552.9879203E-431.808778
GTATAGA552.993113E-431.7993621
GGCATAG802.457864E-630.6159431
AGGCGTG703.47343E-530.00000219
GGTCGCC604.957926E-429.175364
GGCGTGC604.96653E-429.16666820
GATAACG500.00719324428.00000211
TATTGTG500.00720364827.9917145
TCGCCGT657.9037104E-426.93902266
GTCGCCG657.917402E-426.93104765
GAGTATA657.958595E-426.9071521
GCGAGGC808.6277876E-526.25000216
Produced by FastQC (version 0.11.2)