FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_8_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_8_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742780
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG11890.16007431540967715No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC11380.1532082177764614No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAGG657.964587E-426.9184932
AGGGGGG2150.026.05084670
AAGGGGG2750.020.36702569
TTATAGC900.005280335419.4411333
CTAGGCC1107.482279E-419.087664
GTCCTAG950.00720693818.4216381
GTACTTA1602.6046915E-517.5005551
TATATAC1000.00969673517.4970233
TATAGTA1000.00969673517.4970232
TAGTACA1000.00969673517.4970234
ATACTGC1200.001341992817.497026
CTAGTGC1200.001341992817.497023
GTATAGA1250.001761427416.8005311
GTGTAAT1906.114051E-616.5794751
GTACCAA1503.1564885E-416.333851
TAGAGAG3005.5479177E-1016.3305535
TTATACT1503.1613524E-416.3305534
TGGACCG1300.002292376716.1510965
AGTAAGC1300.002293407716.150018
CTACACT1554.0579896E-415.8037614
Produced by FastQC (version 0.11.2)