FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_7_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_7_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271354
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC5550.204529876102803No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG4630.17062582456864464No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT3750.13819586223162364No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2820.10392328839818098No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC2820.10392328839818098No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTAG200.00696271452.4687543
TAGCTAC200.006972872752.44941714
AGCTACC350.001260032139.96146415
AGTAGGG400.002410130835.017963
CACCCGG400.002412313835.01143632
GTAAGGT400.002423253134.9791684
GTATAGC450.004311376731.098331
GTCTAAA604.986357E-429.1546821
GTATAAA755.5881923E-527.9884971
ATAGCAG500.007221768627.9833373
GCACAGT1003.938752E-420.9875036
GGCATAG850.00377624920.59496549
ATAAACC1055.473701E-419.9880983
TGGGTTC1000.00968447817.49603522
TCAACGT1250.001769286616.78381513
GACACCG1300.0022938416.1442345
AAAGGGG1554.0308633E-415.81161970
ACTGCTC1350.002957791315.5405688
CTGCTTG2957.657945E-915.43161768
CAGAACC1450.004746257314.474144
Produced by FastQC (version 0.11.2)