FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_6_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_6_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333354
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC4150.12449228147854834No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG4070.1220924302693233No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3730.11189306263011693No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTA501.7085508E-434.9976732
AGGGGGG851.0603617E-732.94392870
TATTATA703.485068E-529.9980072
TTAGGTA500.007205477527.9981383
TAGGGAG700.00122520224.9983395
AAGGGGG1253.074867E-622.40523569
TTAGAGA1102.9169243E-522.2712464
TAAACCG800.002664239821.8735495
TAAGACT800.002664239821.8735494
TACTTCG850.00379324920.5806927
GTACCGT1205.7050187E-520.415316
GGACTGG1055.4578244E-419.9986726
TGGACTG1257.809085E-519.5986985
TTAGGCA900.005266614719.44606840
GATTTAG900.005266614719.4460681
GTCTTGC900.005266614719.4460681
TTTAATA900.005271186619.4431512
GGCATAG950.007209128718.41706743
CATAGGC950.00721537218.41430345
TCCACCG1150.001006423318.25965749
Produced by FastQC (version 0.11.2)