FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_5_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_5_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72770
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG1080.1484128074756081No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC900.12367733956300674No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT790.10856122028308368No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG740.10169025697402775No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCCC150.002212789470.02959436
CAACCCA150.002218841369.981434
GGTATAT150.002218841369.981431
TCGTTGC200.00691441552.52219860
CAGTACA200.00691441552.52219850
ACTATGA200.006933271452.4860762
GCTGGAC200.006933271452.4860763
CTTGGGT200.006933271452.4860764
GTTTTAA200.006933271452.4860761
TAGCTTT350.001249656139.989396
GGCATGA2203.3287506E-919.0989870
AGGCATG2305.7789293E-918.26858969
TGATCTC1000.00958249917.50739970
TAGGCAT2858.123243E-814.743072568
CTAGGCA3404.7730282E-813.38801167
GACTAGG4551.4242687E-811.5433470
ACTAGGC3701.93525E-611.3561566
AGACTAG5251.16804586E-710.00422869
GAGACTA5754.3866203E-79.13429568
CGAGACT6351.8318788E-68.27121267
Produced by FastQC (version 0.11.2)