FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_4_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_4_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436630
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC6490.14863843528845933No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG5290.12115521150630969No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5210.1193229965874997No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT4840.11084900258800356No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGGT200.006945475552.50704629
GACCGTG500.00721695527.990937
GCAGCGT1101.0153253E-625.452131
TCTAACC851.3100823E-424.697883
TTAGGCA1202.161294E-623.33646440
TATACCA750.001834673923.3257755
GTATAAG1003.936846E-420.99319815
CCTTTTA850.003789137420.586281
TCTACAT1408.255098E-619.993523
CCAACTA1257.8316545E-519.5936513
GCAACGC900.00528161119.43814715
CATTAGA900.00528161119.4381472
TACCGTC900.00528161119.4381477
CCTTATT1501.4971818E-518.660622
GCATAGG1702.1155738E-618.52765344
TATTATA950.00721709818.4150852
TACTCTC950.00721709818.4150855
CCGTGTG1150.001008847518.2549559
GCCTAGA1000.00968632517.4983391
CAGCGTC1401.8669543E-417.4943312
Produced by FastQC (version 0.11.2)