FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_40_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_40_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772731
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG19770.2558458247436689No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC19260.24924585657880943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG1352.3233952E-723.3220115
CCTAGCT800.002663858221.8785433
GCCTAGC850.003786103820.59156632
ATAGGCA3750.018.67210658
TAATACG950.007212552718.4192644
CTATAGA1000.00968216617.50171
CTATTAC1000.00968216617.50171
TGTATAA2005.1713687E-717.49150714
GTGTATA1803.6722486E-617.49150713
GTATAAC1250.001760742716.8016321
TAGTACT2351.542594E-716.3813884
GTATAAT1300.002288399316.1554151
TTACACC1300.002291369316.1522774
GGCATAG4800.016.0463455
TTAGGCA3301.2551027E-1015.90857746
GCAGCGT2009.94593E-615.7515291
AGGGGGG2251.7568927E-615.55908170
GTATAGA1350.002943528115.5570661
TATGCCG4550.015.38810357
TATAAGA2051.2637756E-515.35839616
Produced by FastQC (version 0.11.2)