FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_3_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_3_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321354
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC4130.12851870522850192No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG4040.12571805547775972No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT3650.11358190655787698No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGAC200.006951106252.4931143
CCCTACA400.00241840434.995412
AAGGGGG1500.032.68272469
TATATAC703.4862212E-529.9960673
GCACAGG657.952884E-426.9195446
AGGGGGG1850.026.4995170
AGACTGA901.926379E-423.3302756
TATATAT750.001831568523.330272
ATTACAC952.7778317E-422.1023643
CTCAGAG952.7778317E-422.1023642
TCTTAAA850.003787788820.58553342
CTGGACC1205.707043E-520.4139884
ATTCTAG900.00526801119.4449211
ATAGGCA900.005272754519.44189546
AAAGGGG2607.094059E-1118.8554268
TATAAGA950.007205030418.4186362
ATATATA1150.001005701818.2613161
AGGCATA1602.600883E-517.49770548
GCATAGG1200.001339396717.49770444
GGCATAG1250.001760410716.79779643
Produced by FastQC (version 0.11.2)