FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_39_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_39_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943312
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC13550.14364282443136522No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG11360.12042675170039181No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAG800.002668355621.8727951
GTATAGG1451.1177926E-519.30841
GTATACG1150.001008516818.2590311
TATACTT1953.9608312E-717.9431045
AGGGGGG2357.892595E-917.87907270
CCCCTAT1803.6605343E-617.4982381
TATGTAG1200.001343640517.4945265
GTGTTAG1653.397581E-516.9679891
GTCTATG1653.397581E-516.9679891
CTATGGT1250.001765969316.7947444
GTATGAG1503.1608093E-416.3316881
CTATAAG1300.002295176716.1487942
TAGGCAT3950.015.94945147
TAAATAC2009.98935E-615.7450743
TGTAGGC2452.485358E-715.7093722
TTAGACC1350.002952228815.5506913
TTAGAAC1807.1678005E-515.5506913
GTGTAGG2051.2597669E-515.3643051
ACTGCCC2302.2270451E-615.2094068
GTATAAT1901.13454276E-414.7353571
Produced by FastQC (version 0.11.2)