FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_38_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_38_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797957
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC15200.19048645478390439No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG12510.156775365088595No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAAG808.675277E-526.2464981
GTATTAA1055.4694636E-419.9973321
CTAGGGT1301.0602408E-418.8389174
TAGGTAG950.007224710718.4139775
TAGATAG950.007224710718.4139775
TGTATGG1150.001010252318.2538552
TATACAG1150.001010252318.2538555
TAGGGTG1000.00970929617.4932795
TAATACA1906.139433E-616.572584
GCAGCGT1704.3881817E-516.4683911
TGTAATA2351.5478327E-716.3766882
TACCGTG1300.00229585316.1476427
ATATAGA1350.002948168715.553481
TATAAGC1350.002953098915.5495822
TACACAG1859.058446E-515.1293235
GTATAAC1400.003755912414.9979981
ACACAGG1400.003762181614.994246
GTAATAC1400.003762181614.994243
AATACCG1656.5360486E-414.8427835
ATACACT1656.5360486E-414.8427834
Produced by FastQC (version 0.11.2)