FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_37_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_37_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005415
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC62890.6255128479284673No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG54040.5374894943878896No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCATAGG13960.1388481373363238No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCAAAGC10440.10383771875295275No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG2451.8189894E-1221.41963615
TTAGGCA7400.020.333446
GTATATA1558.76953E-720.3171371
TAAGCCA8550.020.05410446
TAATAGG2053.250352E-818.7754571
GTATAGT1702.124676E-618.5244461
TTAACCC1702.126697E-618.5226063
GCAGCGT2851.2732926E-1118.4161171
CTATACT1351.4161979E-418.1414834
TGTATAA3153.6379788E-1217.77036314
GTGTAAC1401.8692094E-417.4953121
ACTAGAC1602.6151289E-517.493573
GTGTATA3400.017.492713
ATAGGCA10850.017.4250952
TATATAC2056.6940265E-717.06693
ATAGGCT4950.016.97359570
TACTCCG1653.405725E-516.9634635
CAGCGTC3104.9112714E-1116.9301032
CGTATAT1250.001766722516.79382917
TATACTG2108.6127693E-716.6605435
Produced by FastQC (version 0.11.2)