FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_36_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_36_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813093
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC17370.21362869930008008No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG15440.18989217715562673No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAC952.7907518E-422.0931643
TACCCCG800.002674969821.8630285
TATACTG1656.833034E-821.200515
TTAGTAC1055.484869E-419.9890543
TTATACT2104.294452E-818.32334
GTTATAT1351.413062E-418.1460271
GTATTAT1551.9854357E-518.0624051
CGCCGTG1200.001338896517.5033431
TTACACT2207.3101546E-817.4904214
TTAGACA1653.409693E-516.960414
TAGAGTG1704.4039385E-516.4615735
TTAGGCA2803.8871804E-916.255140
GTGTTAG1300.002291810516.1519581
TAGACCG1300.0022984116.1450025
CGTATGC4650.015.8133655
GCATAGC1554.0417575E-415.81238762
GTTCTAG2009.967416E-615.7481591
ATAGGCA4500.015.56235552
TATGCCG4800.015.31825157
GTATATC1605.181315E-415.3041193
Produced by FastQC (version 0.11.2)