FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_35_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_35_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658903
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC11480.17422898362884975No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG11280.1711936354820057No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATAA1705.456968E-1226.75263614
GTGTATA1705.456968E-1226.75263613
GTATAAG1757.2759576E-1225.98827415
TTAATGT750.001835397123.326354
CTACTAA900.005279020419.4415781
ATATATA2002.4416295E-819.247161
GCGTCAG2053.2465323E-818.7748664
AGTACCG950.00721987318.415545
GGGCGTT950.00722619818.41274511
CTAGACT1150.001009422718.2554054
TATATAC1150.001009422718.2554052
CATAGGC2901.8189894E-1118.10902651
GTCCTAA1200.001341570217.497421
CCTGCCT1200.001344302917.4921078
GTATAGA1452.4400798E-416.8940581
GTATTAT1250.00176325516.7975221
AGTATGC1250.0017668416.7924238
ATAGGCA2954.3291948E-1016.61654952
ATGTGTA2753.088644E-916.53799211
TTAGACT1300.002293983216.1490124
Produced by FastQC (version 0.11.2)