FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_34_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_34_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979777
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC13840.14125663288687118No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG12520.12778417946124476No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2850.023.34079270
TTAAGAC1253.1073832E-622.3865763
CTTAGAG850.003794568420.5843011
CCTAGAG1055.4860057E-419.9890352
GTGTATA2801.0913936E-1118.73876413
CTATAGA950.007217813318.4175341
GTATAAT1351.4142398E-418.144681
GTATAAG2901.8189894E-1118.09259815
TGTATAA3203.6379788E-1217.48951314
TATAGTG1000.00972287317.4895135
AAGGGGG3950.016.84082269
GTTTATA2108.596362E-716.663481
GTATAGT1704.3919936E-516.4674421
TATAAGA3651.8189894E-1216.29160116
ATGTGTA3552.1827873E-1115.76519511
GTCTAGA3351.5825208E-1015.6686481
TATGCCG6600.015.38841957
ATATAGA1605.168651E-415.3095731
TATAATA2302.2278036E-615.2090492
TCGTATG6450.015.20331554
Produced by FastQC (version 0.11.2)