FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_32_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_32_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936649
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG16320.17423816178739313No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC15550.1660173661638458No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11210.11968197264930619No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAGG750.001837779423.3224052
TATACCG1003.945337E-420.9901665
CTAAGAC1055.482802E-419.9906333
GTATAAG2653.6379788E-1219.80204415
GTGTATA2653.6379788E-1219.80098713
TATAAGA2850.019.63992316
GTACTAG900.005283926619.439491
CCTTAAG950.00722023618.4163591
GGTTTAA1551.9883179E-518.0599161
TTAAGGG1200.001337350117.5067661
CTTAGCT1200.001345032117.4918062
TTACACT2401.0346412E-817.4918064
CTATACT1401.871904E-417.4918044
TATTAGA1401.871904E-417.4918042
GACCGCG1200.001345513517.4908717
TGTATAA3307.2759576E-1216.96084414
TATGTTA1250.00176779516.7921336
TCTACAC2552.1871529E-816.4628753
TTAGGAC1300.00229754616.146283
TAAGACA1755.6416306E-515.9925084
Produced by FastQC (version 0.11.2)