FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_31_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_31_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785930
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC34570.43986105632817174No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG30470.38769356049520953No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9710.12354789866782029No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCATAGG8310.10573460740778441No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT1957.8216544E-1021.5319044
GTATTAG1003.938504E-420.9949421
GTATATA1003.938504E-420.9949421
GTATTAC1558.762254E-720.3176861
CTAGACT1558.7676017E-720.3163934
GCAGCGT2302.8558134E-1019.7778431
CTAGCCC1107.48957E-419.0850964
GTATTGG1301.0590506E-418.8416161
TTATACT1301.0595677E-418.8404164
GTATAAG2607.2759576E-1118.84041615
ATAGGCA3750.018.67048552
TATACTG1551.9885078E-518.0590155
GTATAAT1953.9557563E-717.9443951
TTAGGCA3600.017.50135240
ACGTTAC1000.00968341517.50135230
GGTTAAG1401.8681682E-417.4957851
TATTATA2259.420728E-817.1059022
TATATTG1452.4437613E-416.8914075
TATAAGA2753.085006E-916.54041716
GTAATAC1704.3944856E-516.4655723
Produced by FastQC (version 0.11.2)