FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_30_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_30_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010418
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC20270.2006100445558175No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG14770.14617712669410085No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12600.12470086637411447No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGT1055.4769864E-419.9940034
GCAGCGT2851.2732926E-1118.4182641
CCCATTA10700.017.66874939
TAATAGG2451.33022695E-817.1402631
TAGACAG2259.428186E-817.1059825
GCTCGCT13750.017.04690221
CTGGATA13300.016.84622830
TAGGACC1250.001765932416.7949644
TATTCCC13500.016.3381435
ATAGGAC1300.002295130416.1490023
CCATTAT11500.016.13358540
TGCTCGC15150.015.93344920
GTAGTAA1554.0592116E-415.8040581
TTAATAC1554.063801E-415.8017123
TATAGTA1554.063801E-415.8017122
CTCGCTT14700.015.71035222
ACGCTAT10500.015.67205470
GTCTAAC1350.002949247115.5532011
CGCTTTC15800.015.50398324
TCGCTTT15050.015.344995523
Produced by FastQC (version 0.11.2)