FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_2_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_2_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339756
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC5320.15658295953566678No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG5150.15157936872343683No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT3840.11302228658213542No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3510.10330943382898315No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1201.8189894E-1235.01207770
CATCGCG552.9985045E-431.8010569
ATGTTAA450.00431247831.0989461
CTTAACC450.00431558631.0943663
AGCGGGC450.00431558631.09436610
GTATTAC703.4929977E-529.9882681
ATCGCGA604.9927016E-429.15096910
AAGGGGG1751.9826984E-1024.00828269
TATGTTG750.001836128223.3207745
TAAGACA952.7859502E-422.0933674
GTCCAGG1003.936148E-420.9917891
TAAGGCT850.00377778620.5953466
ACTAGAC850.00379717520.5771543
AACTGCG850.00379717520.5771547
ACAGATA850.00379717520.5771546
GTCTTGG1205.719711E-520.4086821
GCACAAG1055.4700807E-419.9921781
GCACCGG900.005254381319.4540254
AATATAT900.00528579319.4339792
CCATGCC1451.1185675E-519.2999529
Produced by FastQC (version 0.11.2)