FastQCFastQC Report
Tue 29 Nov 2016
H7KKKBGXY_n01_sp29_29_2016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKKBGXY_n01_sp29_29_2016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401044
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG6300.1570899951127557No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC6120.15260170953810553No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4990.12442525009724618No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCCC200.006962671452.473448
ACCCGGT459.218621E-538.86921311
GCATATG450.004300042531.1186542
ACGCAGG500.007209495627.99630727
TCTTAGG500.007218281727.9893252
ATAGGCA906.144852E-627.2322258
GTATTAT808.6646236E-526.2465381
AGGGGGG1251.0912663E-725.21240270
ATATATC700.001227887924.990473
TATTAGA851.3103313E-424.6964632
TATGGTG750.001835025623.3244364
AAGGGGG1454.5659726E-721.73754169
GATATAT850.003789416320.5855221
ACACTGC1205.728074E-520.4063388
TGCCTCA900.005278816419.43945525
TTAGGCA1301.052483E-418.85307552
GTATATA950.00720809818.4186231
CAAGGGC950.007218480618.414034
TATCCTA950.007218480618.414035
AGGTGCG950.007223676418.4117347
Produced by FastQC (version 0.11.2)